ODN 2395 FITC - TLR9 Agonist

FITC-labeled CpG ODN 2395, Class C, Human/Mouse-preferred

ABOUT

FITC labeled CpG oligonucleotide - Human / Mouse TLR9 ligand

CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs). These CpG motifs are present at a 20-fold greater frequency in bacterial DNA  compared to mammalian DNA.

They have been shown to induce a coordinated   set of immune responses based on the activation of immune cells   primarily involved in the recognition of these molecules.

ODN 2395 FITC is a C-class CpG ODN specific for human / mouse TLR9. ODN 2395  FITC can  be used to evaluate CpG ODN cellular uptake and localization  by  confocal laser-scanning microscopy (excitation 495 nm, emission 520  nm)  or flow cytometry.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Source
Synthetic
Target

TLR9

Sequence

5’-tcgtcgttttcggcgc:gcgccg-3’ (22 mer). Note: Bases are phosphorothioate (nuclease resistant), palindrome is in bold.

Molecular weight
7631 g/mol
Working concentration

1-5 μM for cellular assays

Appearance (form)
Lyophilized
Reconstitution buffer
Sterile endotoxin-free water (provided)
Endotoxin

Negative (tested using EndotoxDetect™ assay)

Tested applications

Cellular assays

Quality control

TLR9 activity has been tested using HEK-Blue™ TLR9 cells.

Additional information

Spectral Properties of FITC:

  • Excitation λ max: 495 nm
  • Emission λ max: 520 nm

CONTENTS

Contents

  • Product: 
    ODN 2395 FITC
  • Cat code: 
    tlrl-2395f
  • Quantity: 
    50 µg
Includes:

2 ml of sterile endotoxin-free water

Notes:

ODN 2395 is labeled with FITC at the 3’ terminus.

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • Upon receipt, store at -20 °C
    Stability: The reconstituted product is stable up to 6 months at -20 °C.

    Caution:

    • Avoid repeated freeze-thaw cycles
    • Protect from light

DOCUMENTS

Documents

ODN 2395 FITC

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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