Normocure™
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Cat.code:
ant-noc
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ABOUT
Broad-spectrum Antibacterial Agent for Contaminated Cell Cultures
Normocure™ is a broad-spectrum antibacterial agent highly effective against Gram-positive and Gram-negative bacteria. Therefore, cell cultures contaminated with bacteria from the environment, such as Staphylococcus species [1] and Achromobacter species [2], can be efficiently cured by Normocure™ treatment. Unlike most antibiotics used to treat cell cultures, such as Penicillin-Streptomycin, Normocure™ is also active against multidrug-resistant bacteria. Additionally, Normocure™ has been successfully used in primary cell cultures for contamination elimination to safely obtain induced pluripotent stem cells [3].
Normocure™ is provided as a ready-to-use solution (50 mg/ml). Simply add to contaminated cell cultures at the recommended concentration (100 μg/ml) for 2 weeks. After 3 passages, the bacterial contamination is completely eliminated.
Note: Normocure™ is intended only for bacteria elimination in cell cultures. Not for human or veterinary use.
More information about Normocure™
References:
1. Mirjalili A, et al., 2005. Microbial contamination of cell cultures: a 2 years study. Biologicals. 33(2):81–85.
2. Gray JS. et al., 2010. Got black swimming dots in your cell culture? Identification of Achromobacter as a novel cell culture contaminant. Biologicals. 38(2):273-7.
3. Geuder. et al., 2021. A non-invasive method to generate induced pluripotent stem cells from primate urine. Scientific Reports. 11:3516.
Normocure™ is intended only for bacteria elimination in cell cultures. Not for human or veterinary use.
SPECIFICATIONS
Specifications
50 mg/ml
Elimination of multidrug resistant bacterial contamination
100 μg/ml
Each lot is functionally tested and validated using microbial reference strains. Absence of toxicity is validated on mammalian control cells.
Decontamination period: 2 weeks (3 passages)
CONTENTS
Contents
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Product:Normocure™
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Cat code:ant-noc
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Quantity:100 mg (2 x 1 ml)
Shipping & Storage
- Shipping method: Room temperature
- 4°C for 3 months
- -20°C for long-term storage
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
Normocure™ contains three bactericidal components belonging to different antibiotic families.
They act by inhibiting the protein synthesis or disrupting membrane integrity.
Their targets are different and completely absent in eukaryotic cells.
The cytotoxicity of Normocure™ is low, however, a slowdown of cell growth may be observed. Once Normocure™ is removed from the culture medium, the cells return rapidly to their normal growth rate.
HEK293 cells (3 105 cells/ml) were spiked with a mixture of Gram- non-fermenting bacilli (Pseudomonas aeruginosa, Alcaligenes xylosodans, Achromobacter sp. and Stenotrophomonas maltophilia) at the concentration of 105 colony forming units (cfu)/ml, and were then either left untreated or treated with 100 U/ml penicillin and 100 µg/ml streptomycin or with 100 µg/ml Normocure™, After 4 days at 37°C, 5% CO2, the bacteria were quantified (cfu per ml).
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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