InvivoGen's siRNA Wizard is a software designed to help you select siRNA/shRNA sequences targeting your gene(s) of interest. This program selects siRNA/shRNA sequences that match criteria suggested by studies of RNA interference and which will have the best expression rate in psiRNA vectors.
Two types of searches can be performed to find siRNA/shRNA sequences:
Standard search utilizes a default set of criteria to analyze your gene of interest and provide the best sequences to silence gene expression.
Advanced search lets you manually set the criteria for selecting the sequences against your target gene.
Using your selected siRNA/shRNA sequence, this tool will design two complementary oligonucleotides forming the insert needed to complete InvivoGen's psiRNA vector.
Start Design hairpin insert
Generate a negative control for siRNA/shRNA. This tool will returns a scrambled sequence with the same nucleotide composition as your siRNA/shRNA input sequence.
Start Scramble siRNA/shRNA
For more information and use criterias you can see our siRNA Selection criteria section, or check out the siRNA design Guidelines
You can also read our siRNA/shRNA review on invivoGen website
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