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AhR signaling pathway

Human HepG2 liver carcinoma - AhR-Lucia reporter cells

HepG2-Lucia™ AhR cells are engineered from the human HepG2 hepatoma cell line for the study of the aryl hydrocarbon receptor (AhR) genomic signaling induction (see details), by monitoring the activity of the Lucia luciferase reporter protein.

AhR is a ligand-dependent transcriptional factor widely expressed in barrier tissues. Notably, AhR plays an important role in gut microbiota and the host's immune homeostasis [1].

HepG2-Lucia™ AhR cells, which express endogenous AhR, are highly relevant for the detection/screening of AhR ligands in food or environmental samples [2].

These cells express the secreted Lucia luciferase reporter gene under the control of a minimal promoter coupled with the human Cyp1a1 gene's entire regulatory sequence, which contains six dioxin-responsive elements (DREs).

As a result, these cells allow studying the AhR genomic signaling pathway, by monitoring the activity of Lucia luciferase in the cell culture supernatant when using the QUANTI-Luc™ 4 Lucia/Gaussia detection reagent.

HepG2-Lucia™ AhR cells are resistant to Zeocin®.

Read our review on AhR's key role in intestinal microbiota and immunity.

References

1. Lamas B. et al. 2018. Aryl hydrocarbon receptor and intestinal immunity. Mucosal Immunol. 11:1024-38.
2. Gao J. et al. 2018. Impact of the gut microbiota on intestinal immunity mediated by tryptophan metabolism. Front. Cell. Infect. Microbiol. 8:13.

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