Notification: Reference #hk-rtor-av can only be ordered together with reference #hk-rtor.

Tetracycline repressor-expressing HEK293 cell line

Inducible gene expression in HEK-RepTor™ cells

HEK-RepTor™ cells are derived from the human embryonic kidney HEK293 cell line, a widely used cellular model. These cells were specifically engineered for InvivoGen's TiGer tet-on system.
They are designed for the tetracycline-inducible expression of a gene of interest (GOI), including cytotoxic proteins, such as gasdermin D (see figures).

Description

HEK-RepTor™ cells stably and constitutively express an optimized tetracycline repressor (TetR) construct [1]. They are readily transfectable with a pTiGer plasmid, carrying a gene of interest (GOI), cloned downstream of the tet-inducible CMV-EF1-TRE promoter. GOI expression is induced upon addition of doxycycline, a synthetic analog of the tetracycline antibiotic, leading to the derepression of the CMV-EF1-TRE promoter. Low doxycycline concentration, ranging from 1 to 10 ng/ml, is sufficient for full induction, thus limiting the antibiotic side effects [2].

Key features

Strong and stable TetR expression for minimal GOI leakiness
Readily transfectable with a pTiGer-mcs or pTiGer-reporter plasmid
Strong GOI expression with a low doxycycline dose

Applications

pTiGer-transfected RepTor™ cells are useful for, but not restricted to the following applications:

Controlled expression of cytotoxic genes
Fine-tuned expression of a GOI
Screening of toxic protein inhibitors

RepTor™ cells are functionally validated by transient transfection with a pTiGer-reporter plasmid expressing either SEAP or Lucia luciferase. These cells are resistant to Blasticidin. They are also extensively tested for viability, stability, and absence of mycoplasma to ensure strong and reproducible results.
For your convenience, RepTor™ cells are shipped with 1 mg of doxycycline and 10 mg of blasticidin.

Learn more about InvivoGen's TiGer Tet-on system.

References:

1. Hillen, W., Wissmann, A. (1989). Tet repressor-tet operator interaction. Protein-Nucleic Acid Interaction. DOI: 10.1007/978-1-349-09871-2_7.
2. Luger, AL., et al., (2018). Doxycycline Impairs Mitochondrial Function and Protects Human Glioma Cells from Hypoxia-Induced Cell Death: Implications of Using Tet-Inducible Systems. Int J Mol Sci, 19(5):1504.

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