Notification: Reference #293-cov2-s-av can only be ordered together with reference #293-cov2-s.
Reference #293-cov2-sdf-av can only be ordered together with reference #293-cov2-sdf.

Studying Spike-binding inhibition with InvivoGen's 293-SARS2 Spike Cells

Available upon request:

• 293-SARS2-S-V2-dfur cells (Alpha/B.1.1.7/U.K variant)
• 293-SARS2-S-V3-dfur cells (Beta/B.1.351/S.A variant)
• 293-SARS2-S-V5-dfur cells (Gamma/P.1/BRA variant)
• 293-SARS2-S-V8-dfur cells (Delta/B.1.617.2/IND variant)
• 293-SARS2-S-V11-dfur cells (Omicron/BA.1 variant)

SARS-CoV-2 Spike (D614)-expressing 293 cells

InvivoGen offers human embryonic kidney (HEK)-293-derived cells, specifically designed for COVID-19 studies:

• 293-SARS2-S Cells: with a functional furin cleavage site and recommended for cell-cell fusion assays with ACE2-expressing cells.

• 293-SARS2-S-dfur Cells: with an inactive furin (dfur) cleavage site facilitating improved surface expression and detection for flow cytometry.

They have been engineered to overexpress the original Wuhan‑Hu-1 SARS‑CoV-2 Spike (S) gene, and for optimal expression, the C‑terminal ER-retention signal has been removed [1,2]. Importantly, the Spike protein contains a furin cleavage site that can affect its cellular expression [3].

More details

A crucial step in developing successful COVID-19 treatments is the validation of their efficacy using rapid, reliable and robust platforms. To this end, cell-based assays using Spike-expressing cells provide a more relevant in vivo biological context than biochemical assays (e.g. ELISAs).

Key Features

Stable expression of the original SARS-CoV-2 Spike (D614) protein.
Functionally validated in cell fusion assays with HEK-Blue™ hACE2(-TMPRSS2) Cells (293-SARS2-S Cells) and by flow cytometry (293-SARS2-S-dfur Cells).

Applications

293-SARS2-S-dfur Cells: Screening patient/vaccinated sera and/or monoclonal antibodies against the SARS-CoV-2 Spike by flow cytometry.
293-SARS2-S Cells: Cell fusion assays for screening novel inhibitors of the SARS-CoV-2 Spike-host interaction using InvivoGen's permissive reporter cell lines (HEK-Blue™ hACE2(-TMPRSS2) and A549-Dual™ hACE2-TMPRSS2).

Learn more about InvivoGen's tools for cell fusion assays

➔ 293-SARS2-S-dfur cells have also been generated with the Spike of other variants of concern: Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta (B.1.617.2), and Omicron (BA.1).
These cells have been used to compare the activity of anti-SARS-CoV-2 Spike antibodies. See data

References:

1. Johnson, M.C. et al. 2020. Optimized Pseudotyping Conditions for the SARS-COV-2 Spike Glycoprotein. J Virol 94.
2. Ou, X. et al. 2020. Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat Commun 11, 1620.
3. Coutard, B. et al. 2020. The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade. Antiviral Res 176, 104742.

Clear allRefined by

Products1

1 Search results: 293-cov2-sdf