A PRACTICAL GUIDE TO UNDERSTANDING INFLAMMASOMES
In 2002, the discovery of inflammasomes was a breakthrough in the understanding of how inflammation is initiated.
InvivoGen offers a comprehensive collection of tools to assist your research on these inflammatory signaling hubs.
Inflammation is an innate immune reaction that aims to resolve various types of threats, including microbial infections, allergies, and auto-immune conditions. One major inflammation effector is the IL-1β cytokine, identified in the 1980's. It took two decades to characterize and assemble the different puzzle pieces that lead to this cytokine's secretion. The discovery of inflammasomes was a major breakthrough in the understanding of inflammation initiation. Inflammasomes are cytosolic multiprotein complexes, whose formation is triggered by sensors of pathogen-associated molecular patterns (PAMPs) and danger-associated molecular patterns (DAMPs). Inflammatory caspases may fulfill the function of a sensor and/or mediator of downstream effectors. While inflammasomes are beneficial in clearing microbes, their aberant or excessive stimulation contributes to various pathologies, such as auto-inflammatory disorders, cardiometabolic and neurodegenerative diseases, as well as cancer.
InvivoGen has developed a large collection of cellular assays, ligands, and inhibitors, to assist your research in this limitless field.
SUMMARY:
Inflammasome actors
- Inflammasome sensors
- Adaptors
- Effectors
Inflammasome cellular assays
- Monitoring NLRP3-dependent responses
- Monitoring NLRC4-dependent responses
- Monitoring ASC-dependent responses
- Monitoring CASP-1-dependent responses
- Monitoring CASP-4-dependent responses
Inflammasome activation and assembly
- A "two-step" activation model
- NLRP3 inflammasome
- NLRC4/NAIP inflammasome
- CASP-11 & CASP-4/5 non-canonical inflammasomes
Inflammasome activation & responses at a glance
- Poster
Inflammasome inducers
- Canonical inflammasome inducers
- Non-canonical inflammasome inducers
Pyroptosis and gasdermin D
Gasdermin D cellular assay
- Monitoring GSDMD-dependent responses
Inflammasome Regulation
Priming agents
- LPS
- Poly(I:C)
- Pam3CSK4
Assessing inflammasome activation
- Monitoring ASC-specks in real-time
- Monitoring pyroptotic cell death
- Monitoring IL-1β and IL-18 secretion
Inflammasome inhibitors
- Caspase inhibitors
- Multi-target inhibitors
- NLRP3 inhibitors