TLR 4
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
|---|---|---|---|---|---|---|
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pUNO1-bTLR4 pUNO1 bearing the bovine TLR4 gene |
Show product |
20 µg |
puno1-btlr4
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pUNO1-hTLR4a pUNO1 bearing the human TLR4 gene, isoform_1 |
Show product |
20 µg |
puno1-htlr4a
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pUNO3-hTLR4a pUNO3 bearing the human TLR4 gene, isoform_1 |
Show product |
20 µg |
puno3-htlr4a
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pUNO1-mTLR4 pUNO1 bearing the mouse TLR4 gene |
Show product |
20 µg |
puno1-mtlr4
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pUNO1-pTLR4 pUNO1 bearing the pig TLR4 gene |
Show product |
20 µg |
puno1-ptlr4
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Expression-ready ORF Clones
Alias : TLR4, CD284
Toll Like Receptor 4
TLR4, the first human TLR identified, is the receptor for Gram-negative lipopolysaccharide (LPS). The TLR4 gene was shown to be mutated in C3H/HeJ and C57BL/10ScCr mice, both of which are low responders to lipopolysaccharide (LPS) [1]. However, TLR4 alone is not sufficient to confer LPS responsiveness. TLR4 requires MD-2, a secreted molecule, to functionally interact with LPS [2]. Furthermore, a third protein, called CD14, was shown to participate in LPS signaling, leading to NF-κB translocation. This signaling is mediated through the adapter protein MyD88 but also through a MyD88-independent pathway that involves the (TIR) domain-containing adapter protein (TIRAP) [3].
1. Poltorak et al. (1998). Science, 282(5396):2085-8.
2. Shimazu et al. (1999). J Exp Med, 189(11):1777-82
3. Horng et al. (2001). Nat Immunol, 2(9):835-41
Specifications
Bovine TLR4 (pUNO1-bTLR4)
Genbank : NM_174198.6
ORF size : 2526 bp
Subclone : AgeI - NheI
Human TLR4 isoform 1 (pUNO1-hTLR4a)
ORF size : 2520 bp
Subclone : BspHI - NheI
Human TLR4 isoform 1 (pUNO3-hTLR4a)
ORF size : 2520 bp
Subclone : BspHI - NheI
Mouse TLR4 (pUNO1-mTLR4)
Genbank : JX878358.1
ORF size : 2508 bp
Subclone : BspHI - NheI
Pig TLR4 (pUNO1-pTLR4)
ORF size : 2526 bp
Subclone : AgeI - NheI
pUNO-TLR Contents
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20 ̊C.
Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.
pUNO1 plamids:
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.
Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.
Avoid repeated freeze-thaw cycles.
pUNO3 plasmid:
- 20 µg of lyophilized DNA
- 1 ml Hygromycin B Gold (hygromycin) at 100 mg/ml
Hygromycin B is a hazardous compound. Avoid contact with skin and eyes.
Store Hygromycin B Gold at 4°C or -20˚C.
Avoid repeated freeze-thaw cycles.